This email address is being protected from spambots. Genomics, Annotation, RNA-Seq, Short-read mapping The course is aimed at PhD students, postdoctoral fellows or other scientists with a solid background in bioinformatics and molecular biology. We mined publically available RNA sequencing (RNA-Seq) data using CLC Genomics Workbench 20 (QIAGEN) and identified 283 differentially expressed genes (FDR<0.05, log2FC>1).Participants will be instructed in the genomics workflow and gain understanding of the possibilities and limitations of genome sequencing and sequence analysis. The course is split into two parts:ġ.) Basic training on Genomics Workbench: Data preparation, QC evaluation, read mapping, variant detection, de novo sequencing, genome finishing, de novo assemblyĢ.) Advanced training on Genomics Workbench: Set-up automatic pipeline, RNA-Seq analysis, Chip-Seq and methylation analysis, OTU Clustering and Analysis of Microbial Communities QIAGEN CLC Genomics Workbench QIAGEN CLC Genomics Workbench is a powerful solution that works for everyone, no matter the workflow. Professional trainers from CLC bio will present the Genomics Workbench with a focus on de-novo genome assembly, short-read mapping, and RNA-Seq experiment. The CLC Genomics Workbench is a commercial software for the analysis and visualization of next generation sequencing data. Analyze your RNA-seq and small RNA (miRNA, lncRNA) data with easy-to-use transcriptomics workflows for differential expression analysis at gene and. There are three major advantages of using paired data: Since the mapped reads span a larger portion of the reference, there will be fewer non-specifically mapped reads. A combination of single reads and paired reads can also be used. Single-cell RNA sequencing (scRNA-seq) is the leading technique for characterizing the transcriptomes of individual cells in a sample. This course will teach participants how to use the CLC Genomics Workbench for genome analysis. The CLC Genomics Workbench supports the direct use of paired data for RNA-Seq. On July 22nd there will be two training sessions.Alexander Goesmann, Jochen Blom, Oliver Rupp, Lukas Jelonek, Rolf Hilker (BiGi) Their next training will be in two sessions, the first using the CLC bio platform for sequence analysis of viral isolates, followed by a review of Ingenuity Pathway Analysis (IPA) on host responses. Based on an annotated reference genome and mRNA sequencing reads, the CLC Genomics Workbench is able to calculate gene expression levels. Qiagen will provide no-cost trial access to our platforms, live demonstrations, and customized training. Qiagen is holding an upcoming Zoom training from QIAGEN Digital Insights. The purpose of the tutorial is to help you get acquainted with the CLC Genomics Workbench RNA-seq tool. Learn QIAGEN IPA and CLC Workbench to generate hypotheses and insights for publications and grant renewals
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